Gene delivery

Gene delivery is the process of introducing foreign DNA into host cells. Gene delivery is, for example, one of the steps necessary for gene therapy and the genetic modification of crops. There are many different methods of gene delivery developed for a various types of cells and tissues, from bacterial to mammalian. Generally, the methods can be divided into two categories, viral and non-viral. Virus mediated gene delivery utilizes ability of a virus to inject its DNA inside a host cell. A gene that is intended for delivery is packaged into a viral particle. Non-viral methods include physical methods such as microinjection, gene gun, impalefection, hydrostatic pressure, electroporation, continuous infusion, and sonication and chemical, such as lipofection. It can also include the use of polymeric gene carriers (polyplexes).

Gene therapy using an adenovirus vector

Further information

1. Горбунова В.Н., Баранов В.С. Введение в молекулярную диагностику и генотерапию наследственных заболеваний. С.-Пб., «Специальная литература», 1997, с.287.
2. Dunlap D.D., Maggi A., Soria M.R., Monaco L. Nanoscopic structure of DNA condensed for gene delivery. //Nucl. Acids. Res., 1997, vol. 25, 3095–3101.
3. Park T.G., Jeong J.H., Kim S.W. Current status of polymeric gene delivery systems. // Adv. Drug Deliv. Rev., 2006, vol. 58, 467– 486.
4. Lechardeur D., Verkman A.S., Lukacs G. L. Intracellular routing of plasmid DNA during non-viral gene transfer. // Adv. Drug Deliv. Rev., 2005, vol. 57, 755– 767.
5. Maxfield F.R. and McGraw T.E. Endocytic Recycling. // Nature Rev. Mol. Cell. Biol., 2004, vol. 5, 121–132.
6. Saul JM, Linnes MP, Ratner BD, Giachelli CM, Pun SH (November 2007). "Delivery of non-viral gene carriers from sphere-templated fibrin scaffolds for sustained transgene expression". Biomaterials 28 (31): 4705–16.
7. Article Gene delivery from Wikipedia, the Free Enciclopedia. Available under the license Creative Commons Attribution-Share Alike.

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